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Brought to you by Horizon Discovery, the leader in CRISPR functional genomics offering a large portfolio of CRISPR screening tools, reagents, and cell line generation.

CRISPR Functional Genomics Miniseries

Episode #14: Kevin Hemphill on the Future of Base Editing

In the final episode of this podcast series, Horizon R&D Manager Kevin Hemphill outlines some of the future applications and priorities for base editing technology on the horizon.

Episode #13: Jennifer Harbottle on Base Editing

In this episode, Jennifer Harbottle, Senior R&D Scientist with Horizon, discusses Horizon’s research and resources in the field of base editing.

Episode #12: Steve Smith, the Rise of CRISPR Modulation Techniques

In this episode, Steve Smith, Product Manager with Horizon Discovery, reviews the latest tools in the CRISPR toolbox for gene modulation, activation and suppression.

Episode #11: Nicola McCarthy, CRISPR Screens in Primary Cells

In this episode, Horizon’s Screening Business Unit Manager, Nicola McCarthy, shares some best practices when it comes to running CRISPR screens in primary cells.

Episode #10: Ciaran Lee, Favoring HDR

In this episode, Ciaran Lee shares some insights on the choice of repair pathways in editing experiments and strategies to increase HDR in primary stem cells and other contexts.

Episode #9: Samira Kiani, CRISPR Safety

In this episode, Samira Kiani discusses genetic means of regulating CRISPR to ensure safety and clinical applications in immunomodulation.

Episode #8: Mandana Arbab, Editing Outcomes

In this episode, Mandana Arbab, a postdoc in David Liu’s lab, discusses editing outcomes using CRISPR-Cas9 and base editing and the development of the respective online resources, inDelphi and BE-Hive.

Episode #7: Simplifying CRISPR Knockout Workflows

In this episode, Ryan Donnelly (Horizon Discovery) discusses a user-friendly guide to streamlining CRISPR knockout workflows in cell lines including guide design, delivery and strategy, as well as tips for avoiding common pitfalls.

Episode #6: Jason Sheltzer

Jason Sheltzer (Cold Spring Harbor Laboratory) discusses challenges and best practices to generating gene knockouts in cell lines using CRISPR-Cas9, as well as his group's tour de force application of the technology to reveal stunning discrepancies between drugs that are in clinical trials and their supposed mechanism of action.

Episode #5: Britt Adamson

Britt Adamson (Princeton University), one of the pioneers of single-cell screening using CRISPR going back a few years to her postdoc in the Jonathan Weissman lab at UCSF, discusses technical tips, trends and applications of this extremely powerful approach in functional genomics, and highlights the value of a strong multidisciplinary team.

Episode #4: Omar Abuddayeh and Jonathan Gootenberg

Omar Abuddayeh and Jonathan Gootenberg (McGovern Institute for Brain Research, MIT) offer tips and strategies for minimizing off-target effects using CRISPR-Cas9. We also hear about their exciting work on SHERLOCK and CRISPR diagnostics, one of the highlights of their tenure in the Feng Zhang lab; and get a preview of some new directions now they are independent investigators.

Episode #3:  Stanley Qi

Stanley Qi, Assistant Professor at Stanford University and Associate Editor with The CRISPR Journal, discusses the development and application of CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) in better understanding gene regulation and pathway relationships.

Episode #2:  Cristina Ghirelli

In this podcast, Cristina Gherelli (Team Leader in Immuno Oncology R&D at Horizon Discovery) shares some of the latest tips in screening immune cells, particularly using pooled lentiviral T-cell screens. She also discussed the implications of this new screening application for immuno-oncology and autoimmunity research.

Episode #1:  Alejandro Chavez

In this episode, physician-scientist Alejandro Chavez (Columbia University) discusses the development and features of the CRISPR activation platform, which is commercially available from Horizon. Chavez also offers his views on the breadth of applications of CRISPRa/CRISPRi technology for genetics research.


CRISPR Screening: From target selection to patient stratification

Original Air Date: May 14, 2019

In this webinar, Eric Shifrut, postdoctoral fellow at UCSF, and Benedict Cross, Head of Functional Genomic Screening at Horizon, will present the principles, strategies and experimental outcomes of CRISPR screening, including screening in primary T cells.

Genetic Rocket Fuel: The Landscape of CRISPR Technologies

Original Air Date: September 18, 2019

In this webinar, Dr. Ross Wilson, an alumnus of the Doudna laboratory at UC Berkeley, will discuss this remarkable array of molecular machines, the tools in an expanding toolkit that allows researchers to explore the ever-expanding territory.


Best Practices and Challenges in CRISPR Screening

The remarkable power of CRISPR technology is revolutionizing many areas of molecular biology, but none more so than functional genomics and high-throughput screening. In this engaging roundtable discussion, four distinguished experts in CRISPR technology development and application (Traver Hart, PhD, Martin Kampmann, PhD, Shondra Pruett-Miller, PhD, and Ophir Shalem, PhD) consider some of the best practices and challenges in performing successful CRISPR screens in a wide variety of experimental contexts.